FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7854041
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC97450.077.28521120
GAGCACA153900.045.878677120
AGAGCAC207150.035.15726118-119
AAGAGCA318250.023.602684120
GAAGAGC359950.021.862934120
GGAAGAG440450.018.062777120
TCGGAAG524850.015.928681120
CGGAAGA513800.015.458332118-119
ATCGGAA620000.012.98156118-119
GATCGGA637000.012.588278118-119
AGATCGG715300.012.095454120
CACGTCT23650.010.652519120
TAGATCG165000.010.567563116-117
CTAGATC42750.010.002844118-119
GAGATCG292450.09.226621118-119
CCCCCCC7051.1073329E-49.0903261
AAGATCG249300.08.722711120
TTAGATC60100.08.714766116-117
CAGATCG258950.08.500061120
ACACGTC27650.08.392141120
Produced by FastQC (version 0.11.5)