FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13390612
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC150350.068.281006120
GAGCACA230050.045.218723120
AGAGCAC310150.034.926952120
AAGAGCA473700.022.721481118-119
GAAGAGC524750.020.682364120
GGAAGAG646000.016.904089118-119
TCGGAAG746600.014.525805116-117
CGGAAGA761850.014.362154120
ATCGGAA885050.012.35383114-115
GATCGGA890100.012.260752114-115
CACACGT39400.012.1271105120
AGATCGG971100.011.485334120
CTAGATC53700.010.931499120
TAGATCG219700.010.648712118-119
CACGTCT44000.09.463084120
ACACGTC45300.09.342196120
TTAGATC95350.09.227881118-119
AAGATCG426500.08.943097110-111
GAGATCG334100.08.574498114-115
CGGCTAG7400.00379461058.301686120
Produced by FastQC (version 0.11.5)