FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12562868
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC193900.0114.8204120
GAGCACA325600.067.74552120
AGAGCAC447000.049.6668120
AAGAGCA639550.034.672676118-119
GAAGAGC736050.030.042147116-117
GGAAGAG880400.025.167252116-117
CGGAAGA1051200.020.954807114-115
TCGGAAG1084850.020.296583114-115
ATCGGAA1255300.017.5832112-113
GATCGGA1291100.017.189182112-113
AGATCGG1402400.015.783412110-111
CACGTCT55100.014.448087120
TAGATCG317400.014.274401110-111
CTAGATC74600.014.267872108-109
ACACGTC58250.014.127452120
CACACGT51250.013.962657120
ACGTCTG67200.013.710076120
AAGATCG415800.013.219734110-111
GAGATCG484750.013.083194110-111
CAGATCG478000.012.743965110-111
Produced by FastQC (version 0.11.5)