FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11079884
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC139100.087.05755120
GAGCACA224900.054.960274120
AGAGCAC308850.040.808887120
AAGAGCA462250.027.188385118-119
GAAGAGC522000.024.363699120
GGAAGAG642150.019.547945116-117
TCGGAAG756400.016.595234114-115
CGGAAGA748950.016.59291118-119
ATCGGAA890400.014.136238116-117
GATCGGA907000.013.881443112-113
AGATCGG981350.012.771833114-115
TAGATCG200400.011.792002114-115
CTAGATC53500.011.07811114-115
ACACGTC43200.010.558089120
CACGTCT43400.010.509435120
CACACGT39000.010.330684120
AAGATCG403800.010.170651112-113
TTAGATC85800.010.100326120
GAGATCG338200.010.053999112-113
CAGATCG328050.010.04005110-111
Produced by FastQC (version 0.11.5)