FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12930980
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC126300.066.27046120
GAGCACA199850.043.638466120
AGAGCAC265900.033.63018120
AAGAGCA429550.022.165228120
GAAGAGC472850.020.039228120
GGAAGAG575850.017.222862120
TCGGAAG681350.014.450993118-119
CGGAAGA690900.014.3172245120
GATCGGA804650.013.141906120
ATCGGAA797350.012.528153120
AGATCGG887450.011.879111120
TAGATCG214400.010.935815118-119
CTAGATC56200.010.0542965118-119
GAGATCG341550.09.40627120
TTAGATC84000.09.368087120
CAGATCG343700.09.082523120
AAGATCG271300.08.211322118-119
CCTAGAT22950.08.065493118-119
GCCTAGA10252.2671958E-47.613813120
CACACGT39550.07.564081120
Produced by FastQC (version 0.11.5)