FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13180707
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC123100.053.18109120
GAGCACA176050.035.140034120
AGAGCAC220100.028.80689118-119
GAAGAGC386700.019.086409120
AAGAGCA366600.018.581545120
GGAAGAG476850.014.92293118-119
TCGGAAG538600.013.703517120
CGGAAGA549850.013.274285118-119
GATCGGA634050.012.388056120
ATCGGAA646000.012.100204120
AGATCGG708850.011.731596120
TAGATCG192400.010.345727120
CTAGATC51350.09.148098114-115
AAGATCG284150.08.695302120
TTAGATC85900.08.386239120
GAGATCG277650.08.238847120
CAGATCG233700.07.2851653118-119
ATAGATC106550.06.509889118-119
CCTAGAT23000.06.3191047120
ACACGTC42800.06.20099120
Produced by FastQC (version 0.11.5)