FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11571473
Sequences flagged as poor quality0
Sequence length63-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA320800.010.435783120
GCCGTAT416300.09.555037120
AAAAAAA971350.09.505788120
CCGTATC375700.09.134401120
GGTCGCC543050.08.805291120
GTATCAT342150.08.8026285120
CGCCGTA465050.08.643714120
TGGTCGC559950.08.475249120
GTGGTCG603800.08.406253120
GGTGGTC656400.08.171357120
ATCATTA350900.08.035996120
TCGGTGG753700.07.888608120
TCGCCGT534850.07.8746333120
CGGTGGT720000.07.6412086120
GTCGCCG555550.07.559623120
CTCGGTG816650.07.449502120
TCATTAA312950.07.4192705120
ATCTCGG910400.07.1502743120
TCTCGGT848600.07.09499118-119
GCGTCGG81900.07.0325456120
Produced by FastQC (version 0.11.5)