FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11571473
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC127800.069.55212120
GAGCACA196600.044.13678120
AGAGCAC255750.034.83835120
AAGAGCA415900.022.609608120
GAAGAGC431150.021.13529118-119
GGAAGAG513550.017.785233118-119
CGGAAGA582350.015.774617116-117
TCGGAAG585450.015.4926195116-117
CACGTCT40400.013.609412120
ATCGGAA681900.013.446384114-115
GATCGGA697600.013.042812114-115
CACACGT32050.011.876593120
ACACGTC38800.011.808854120
AGATCGG784550.011.705084112-113
TAGATCG167100.011.041865112-113
ACGTCTG49700.09.928137120
AAGATCG329900.09.584432118-119
CTAGATC45850.09.292521110-111
GCACACG61350.09.19182120
GAGATCG261950.08.960906120
Produced by FastQC (version 0.11.5)