FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13565164
Sequences flagged as poor quality0
Sequence length51-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCTC237400.09.29967120
TAGATCT237100.08.982499120
AGTGTAG334150.08.941046120
GAGTGTA369250.08.676033120
GATCTCG238450.08.554252120
AGAGTGT408850.08.37862120
TGTAGGG713450.08.139772120
GTGTAGA346550.07.9979095120
GGGAAAG615150.07.9776087120
TAGGGAA642050.07.961065120
GTAGGGA666750.07.864096120
GTAGATC341150.07.755211120
GTGGTCG161600.07.573371120
AAGAGTG489900.07.5067763120
ATCTCGG236600.07.5054617120
TGGTCGC145800.07.4476876120
GTGTAGG786350.07.438565120
CTCGGTG225150.07.407528120
TCGTGTA874800.07.283098120
CGTGTAG821950.07.131118118-119
Produced by FastQC (version 0.11.5)