FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12552037
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC177300.078.957695120
GAGCACA279350.049.164455120
AGAGCAC370150.037.67133118-119
AAGAGCA548850.026.59323120
GAAGAGC618900.022.798088118-119
GGAAGAG724750.019.636072118-119
CGGAAGA846450.016.682404116-117
TCGGAAG874800.016.549917120
ATCGGAA1023100.014.179846118-119
CACGTCT54900.013.9502535120
GATCGGA1059050.013.590724118-119
CACACGT50050.012.800775120
AGATCGG1184100.012.307842116-117
TAGATCG260800.011.831165120
ACGTCTG68150.011.5621605120
ACACGTC60700.011.040178118-119
CTAGATC72850.010.614126116-117
TTAGATC91350.09.997294110-111
CCTAGAT24700.09.689688118-119
AAGATCG443800.09.599297118-119
Produced by FastQC (version 0.11.5)