FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11699186
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC169100.090.7367120
GAGCACA276000.056.75691120
AGAGCAC358900.042.92023118-119
AAGAGCA520100.029.12325118-119
GAAGAGC596400.025.665964116-117
GGAAGAG722750.020.851196116-117
CGGAAGA840150.018.343704114-115
TCGGAAG861900.017.456734114-115
CACGTCT58700.015.601269120
ATCGGAA1005700.015.428003112-113
CACACGT54100.015.071932118-119
GATCGGA1042300.014.593384112-113
ACACGTC58900.014.389222118-119
AGATCGG1136950.013.735965110-111
TAGATCG300150.013.021133110-111
ACGTCTG68000.012.995021120
CTAGATC61250.012.460388108-109
GAGATCG416400.010.649039114-115
TTAGATC88100.010.57748118-119
GCACACG70000.010.557863116-117
Produced by FastQC (version 0.11.5)