FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13864791
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC102600.056.120583120
GAGCACA146800.039.480446120
AGAGCAC194500.030.833426120
AAGAGCA335650.019.329493120
GAAGAGC370750.018.178448120
GGAAGAG505900.013.832115120
TCGGAAG564900.012.632519120
GATCGGA627150.012.0810175120
CGGAAGA612650.011.719839120
ATCGGAA654850.011.233657120
AGATCGG683700.011.183022120
TAGATCG174050.09.97892120
CTAGATC44100.09.418149116-117
TTAGATC78850.08.539462120
AAGATCG357050.08.371688120
GAGATCG233950.07.692924120
CAGATCG206700.07.6262884118-119
TAAGATC157850.06.6975074120
CCTAGAT21350.06.484627116-117
TGAGATC110850.06.0187283110-111
Produced by FastQC (version 0.11.5)