FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12197555
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC132200.066.03952120
GAGCACA191450.043.03586120
AGAGCAC263300.034.227314120
AAGAGCA412750.021.532675120
GAAGAGC460000.019.56294120
GGAAGAG589450.016.13336120
TCGGAAG668300.013.9604435118-119
CGGAAGA690300.013.615145118-119
ATCGGAA796800.012.206252120
GATCGGA782850.012.130946120
AGATCGG858450.011.299137120
TAGATCG189700.010.702928118-119
AAGATCG385400.09.58457120
CTAGATC50250.09.18951114-115
TTAGATC83200.08.659137120
GAGATCG282900.08.496525118-119
CAGATCG268250.08.154775120
CCTAGAT16850.07.579891116-117
CAAGATC225250.06.9492564120
GCTAGAT21200.06.7971377114-115
Produced by FastQC (version 0.11.5)