FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13114299
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC152850.080.228905120
GAGCACA255850.047.072083120
AGAGCAC344450.035.60303118-119
AAGAGCA518000.023.991669120
GAAGAGC584050.021.226408118-119
GGAAGAG694550.017.912905120
CGGAAGA823750.015.154333116-117
TCGGAAG819600.015.046853116-117
ATCGGAA961200.013.086408120
GATCGGA995300.012.734581120
AGATCGG1105700.011.444905118-119
TAGATCG236950.010.759466118-119
CACACGT40300.010.727716120
CTAGATC56500.010.142326116-117
CACGTCT42700.010.124752120
TTAGATC91150.09.225653112-113
AAGATCG428250.08.952748110-111
GAGATCG431200.08.681915116-117
CAGATCG378650.08.020192114-115
ACACGTC50250.07.7841377120
Produced by FastQC (version 0.11.5)