FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15787087
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC193300.076.3214120
GAGCACA312150.050.906357120
AGAGCAC424750.036.72955120
AAGAGCA636500.024.955717118-119
GAAGAGC729150.021.72689120
GGAAGAG894800.017.782623118-119
TCGGAAG1061600.015.267085120
CGGAAGA1055200.014.936477116-117
ATCGGAA1244850.013.006474118-119
GATCGGA1263400.012.812776118-119
AGATCGG1399500.011.648584116-117
ACACGTC64400.010.811849120
TAGATCG296900.010.54221118-119
CTAGATC75950.09.896296112-113
TTAGATC120250.09.688711120
CACACGT58200.09.594591120
AAGATCG576350.09.456324116-117
CACGTCT59250.09.192312118-119
GAGATCG458800.09.152883110-111
CAGATCG462250.09.05266120
Produced by FastQC (version 0.11.5)