FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12238849
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC136350.068.94176120
GAGCACA210050.045.383892120
AGAGCAC288000.036.371174120
AAGAGCA439000.024.16304120
GAAGAGC499050.021.468225120
GGAAGAG616950.017.591423120
TCGGAAG736350.014.815635118-119
CGGAAGA749650.014.654448120
ATCGGAA863900.013.461272120
GATCGGA885400.012.669858120
AGATCGG998000.011.5395775118-119
CTAGATC59900.011.296425120
TAGATCG222150.010.511557118-119
AAGATCG404150.09.569596120
TTAGATC86950.09.308033120
GAGATCG371700.08.852313120
CAGATCG347800.08.564154120
CCTAGAT20600.08.534434116-117
ACACGTC47950.08.439348120
CACACGT41550.08.30233120
Produced by FastQC (version 0.11.5)