FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12600860
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC132000.064.20452120
GAGCACA201300.043.027683120
AGAGCAC263800.033.39411120
AAGAGCA416800.021.613972120
GAAGAGC463000.020.373856120
GGAAGAG576650.017.16131120
CGGAAGA685850.014.860187120
TCGGAAG673700.014.822823118-119
GATCGGA800700.012.784901120
ATCGGAA791900.012.553509118-119
AGATCGG884100.011.346118120
TAGATCG206150.010.885947120
CTAGATC51300.09.714063116-117
AAGATCG399750.09.249005114-115
CCTAGAT18101.8189894E-128.881484120
TTAGATC91750.08.795372116-117
GAGATCG301150.08.71167120
CAGATCG273450.07.6423965120
TAAGATC149300.07.5801353120
CACACGT32500.07.3205295120
Produced by FastQC (version 0.11.5)