FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15143018
Sequences flagged as poor quality0
Sequence length50-126
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC126100.056.951122120
GAGCACA200550.035.80921120
AGAGCAC253100.028.50954118-119
AAGAGCA395700.019.957546120
GAAGAGC453550.018.015701120
GGAAGAG550600.014.625465118-119
TCGGAAG609400.014.225263120
CGGAAGA652550.012.960366120
GATCGGA746600.012.478013120
ATCGGAA730000.012.045668120
AGATCGG853050.011.679613120
TAGATCG203950.010.893215120
CTAGATC51800.010.391972118-119
TTAGATC82500.08.825585120
GAGATCG413350.08.536439120
AAGATCG303800.07.947958120
CAGATCG359500.07.0108023118-119
TCTAGAT30100.06.9261184116-117
GCTAGAT26400.06.836057116-117
CCTAGAT20850.06.71565116-117
Produced by FastQC (version 0.11.5)