FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11749975
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC153450.069.61671120
GAGCACA236850.045.745403120
AGAGCAC310300.034.80576118-119
AAGAGCA478750.022.48701118-119
GAAGAGC507750.021.250494116-117
GGAAGAG611200.017.399157116-117
CGGAAGA712600.015.267906114-115
TCGGAAG721550.014.709588114-115
CACGTCT49900.013.025043120
ATCGGAA852850.013.004399112-113
CACACGT41800.012.985099118-119
GATCGGA855100.012.578003112-113
ACACGTC48350.012.012535118-119
AGATCGG957150.011.674007110-111
TAGATCG231350.010.8041525118-119
CTAGATC52500.010.404623110-111
ACGTCTG61550.09.661003120
TTAGATC87800.09.489524118-119
GAGATCG354050.09.37415116-117
AAGATCG319050.09.318834120
Produced by FastQC (version 0.11.5)