FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16051394
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC64900.017.8422533
CCGATCT66200.016.1324064
AAAAAAA1901000.014.107992120
GCGTCGG97350.012.819385120
CGATCTA19650.012.2133435
TCTCGGG40850.010.428122120
TCATTAA694450.09.883821120
CATTAAA658200.09.662461120
CGTATCA920450.09.347355120
CGCCGTA1137450.09.288955120
GTATCAT878750.09.162774120
GGTCGCC1408700.09.161396120
CCGTATC1030900.08.875505120
ATCATTA777550.08.758048120
TCGCCGT1247300.08.701767120
GCCGTAT1084650.08.629284120
TATCATT897350.08.518191120
GTCGCCG1334850.08.423196120
GGTGGTC1725450.08.3489065120
TGGTCGC1492500.08.289228120
Produced by FastQC (version 0.11.5)