FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16051394
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC224400.0119.79309120
GAGCACA384450.070.521286120
AGAGCAC547850.049.79248118-119
AAGAGCA811000.033.69388118-119
GAAGAGC946200.028.884102116-117
GGAAGAG1172100.023.393665116-117
CGGAAGA1402700.019.59951114-115
TCGGAAG1434750.019.111147114-115
TCCGATC65200.018.0844423
ATCGGAA1619800.016.923128112-113
CCGATCT68700.016.7015824
GATCGGA1656200.016.55119112-113
AGATCGG1796650.015.171435110-111
TAGATCG445200.013.960265110-111
CTAGATC96300.013.554528110-111
GAGATCG648700.013.006837110-111
CGATCTA20350.012.7316975
CAGATCG617300.012.535109114-115
TTAGATC147950.012.167091108-109
AAGATCG415900.012.143728110-111
Produced by FastQC (version 0.11.5)