FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13064634
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGAA319250.010.110079120
AGTGTAG156400.09.743167120
GTAGGGA330350.09.425323120
GTGTAGG409750.09.048418120
AGAGTGT191300.08.969211120
TGTAGGG365250.08.968205120
TCGTGTA467850.08.553077120
TAGATCT114950.08.454914120
GAGTGTA164800.08.445666120
AAGAGTG221900.08.300117120
CGTGTAG432850.08.232887120
AGCGTCG641750.08.020927120
GTCGTGT498450.07.955784120
GTAGATC189550.07.6593995120
AGGGAAA387500.07.53981120
GTGTAGA172000.07.49917120
GAGCGTC701350.07.459073120
CGTCGTG578800.07.4110656120
AGAGCGT745200.07.261676120
AAGAGCG802000.07.18873120
Produced by FastQC (version 0.11.5)