FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13064634
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC114950.045.078712120
GAGCACA167550.032.109264120
AGAGCAC199650.027.814943120
AAGAGCA323000.018.438585120
GAAGAGC344850.017.018965120
GGAAGAG430550.014.522883120
TCGGAAG480700.013.188058120
CGGAAGA511100.012.476316120
GATCGGA593400.012.196128120
ATCGGAA590900.011.786736120
TAGATCG167450.011.276381120
AGATCGG665400.011.183473120
CTAGATC46450.010.595865118-119
TTAGATC75400.09.031842120
AAGATCG313300.07.785578120
GAGATCG301700.07.0691786118-119
CCTAGAT21350.06.3794003118-119
TCTAGAT31850.06.2200747118-119
TAAGATC120500.06.113852120
CAGATCG293100.06.0620728120
Produced by FastQC (version 0.11.5)