FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14122256
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC188750.080.3436120
GAGCACA287400.047.966625120
AGAGCAC398400.036.772614120
AAGAGCA593350.025.014482120
GAAGAGC690050.021.54398118-119
GGAAGAG837000.018.077114120
CGGAAGA988200.015.252935116-117
TCGGAAG1009450.014.666071118-119
ATCGGAA1162500.012.921899114-115
GATCGGA1181450.012.731254120
AGATCGG1289450.011.629079112-113
TAGATCG263500.010.885331120
CTAGATC69450.09.9796715116-117
AAGATCG622950.09.968765120
CACGTCT54050.09.902501120
GAGATCG389100.09.734728120
TTAGATC116350.09.613792114-115
TAAGATC240800.08.991898110-111
CCTAGAT21800.08.498714120
CACACGT52550.08.487634120
Produced by FastQC (version 0.11.5)