FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15007962
Sequences flagged as poor quality0
Sequence length52-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT232900.09.408871120
GAGTGTA346400.08.891062120
AGAGTGT382100.08.76741120
GCGTCGG80950.08.454777120
AGTGTAG314100.08.395041118-119
AGATCTC232600.08.311134120
GTGTAGA324200.08.296199120
AAGAGTG436450.07.881961120
GATCTCG235600.07.746624120
GTAGGGA640150.07.690353120
GTAGATC325900.07.663429120
TAGGGAA615800.07.6239705120
TGTAGAT368700.07.5717096120
TGTAGGG680150.07.467579120
GTGTAGG737400.07.4414434120
CGTGTAG782000.07.377866120
ATCTCGG233050.07.2131186120
TCGTGTA831250.06.969631120
TCTCGGT209800.06.8964515120
GGGAAAG648200.06.6315975120
Produced by FastQC (version 0.11.5)