FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13866202
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC186950.075.772316120
GAGCACA293900.045.93348120
AGAGCAC382900.036.847668120
AAGAGCA576400.024.956976120
GAAGAGC667400.021.330193118-119
GGAAGAG768900.018.601772118-119
CGGAAGA901050.015.94921120
TCGGAAG928550.015.446345120
CCCCCCC3451.8209521E-514.7804761
ATCGGAA1080400.013.537595120
GATCGGA1099100.013.262157120
AGATCGG1222400.012.29527120
TAGATCG288300.011.508231116-117
CACGTCT68450.010.761329120
TTAGATC102100.010.658906116-117
CTAGATC74300.010.557821114-115
GAGATCG470050.010.125844120
CAGATCG428600.09.859677118-119
CCTAGAT23100.09.792216110-111
GCCTAGA8800.09.640235108-109
Produced by FastQC (version 0.11.5)