FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14935106
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC182050.088.329414120
GAGCACA298250.051.997124120
AGAGCAC401300.039.392548118-119
AAGAGCA616200.025.6846120
GAAGAGC684050.023.268435116-117
GGAAGAG821350.019.23404120
CGGAAGA956350.016.730928114-115
TCGGAAG982450.015.987404118-119
ATCGGAA1156550.014.11668120
GATCGGA1168150.013.712521118-119
AGATCGG1277100.012.466132118-119
TAGATCG299650.011.409164116-117
CTAGATC75650.011.106881120
AAGATCG520600.010.233039116-117
GAGATCG390150.09.904418116-117
TTAGATC127850.09.517758116-117
CAGATCG388500.09.33156114-115
TAAGATC234500.09.143207108-109
CCTAGAT26350.08.796551120
CACACGT38650.08.433466120
Produced by FastQC (version 0.11.5)