FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13470480
Sequences flagged as poor quality0
Sequence length50-126
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC118000.053.225445120
GAGCACA184350.036.62919120
AGAGCAC236050.027.913445120
AAGAGCA352700.019.35954120
GAAGAGC416500.016.696215120
GGAAGAG486650.015.41453120
TCGGAAG542750.014.57492120
CGGAAGA588700.013.12729120
ATCGGAA638200.012.612015120
GATCGGA636500.012.438069118-119
AGATCGG737950.011.290984120
CTAGATC47300.010.510822118-119
TAGATCG185600.010.4433975116-117
TTAGATC71650.09.310229118-119
CCTAGAT18900.08.657569112-113
GCTAGAT18850.08.179443116-117
AAGATCG303100.08.097471120
TCTAGAT24050.07.457625118-119
GAGATCG336350.07.2221513118-119
CCCTAGA8703.3255674E-76.871845116-117
Produced by FastQC (version 0.11.5)