FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19673215
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC204700.063.640343120
GAGCACA311650.041.970676120
AGAGCAC402150.032.600353118-119
AAGAGCA628100.021.784496120
GAAGAGC700350.019.21129118-119
GGAAGAG843550.016.157738120
TCGGAAG974600.013.778099116-117
CGGAAGA989050.013.673898118-119
GATCGGA1180200.012.065042120
ATCGGAA1170350.011.781781120
AGATCGG1287900.011.03554120
TAGATCG301400.010.569339120
CTAGATC74750.010.100425120
TTAGATC113400.08.088939116-117
GAGATCG564700.07.9459596118-119
CAGATCG542850.07.265354118-119
CCTAGAT27250.07.169782112-113
AAGATCG453650.07.0879908116-117
CACGTCT67300.07.0853844120
CACACGT63150.06.712011120
Produced by FastQC (version 0.11.5)