FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15367090
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC225550.0118.368355120
GAGCACA379850.068.47819120
AGAGCAC538200.049.542282120
AAGAGCA791350.033.558247118-119
GAAGAGC919450.028.957378118-119
GGAAGAG1124750.023.664124116-117
CGGAAGA1310650.020.40571116-117
TCGGAAG1359650.019.577858114-115
ATCGGAA1542000.017.315453114-115
GATCGGA1596100.016.649702112-113
AGATCGG1725100.015.407149112-113
TAGATCG451300.014.180983114-115
CTAGATC106750.013.644451116-117
GAGATCG547350.013.240968110-111
CAGATCG663650.013.115024110-111
TTAGATC143850.012.446899112-113
CCTAGAT36700.012.021702112-113
AAGATCG393450.011.812034110-111
TGAGATC221300.010.819352120
GTAGATC151850.010.718977108-109
Produced by FastQC (version 0.11.5)