FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14786021
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC186200.086.423164120
GAGCACA311400.050.119343120
AGAGCAC423700.038.366714120
AAGAGCA637200.025.538626118-119
GAAGAGC742900.022.639969120
GGAAGAG887850.018.831352120
CGGAAGA1030250.016.373238118-119
TCGGAAG1061600.015.856719120
ATCGGAA1224300.013.769042116-117
GATCGGA1270800.013.381025120
AGATCGG1398000.012.163525120
TAGATCG338400.011.756609120
CTAGATC70100.010.780784118-119
TTAGATC116200.010.277113118-119
GAGATCG536700.010.016182118-119
AAGATCG452900.09.728915120
CAGATCG447900.09.503236120
ACACGTC63050.09.385798120
CCTAGAT25550.09.208763120
CACACGT56650.08.935542118-119
Produced by FastQC (version 0.11.5)