FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12056450
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC153000.074.43033120
GAGCACA244550.046.103027120
AGAGCAC329800.035.641434120
AAGAGCA502950.023.212122120
GAAGAGC566900.021.287548120
GGAAGAG687300.017.936764120
CGGAAGA814450.015.127997118-119
TCGGAAG839250.014.736898120
ATCGGAA980700.012.76091120
GATCGGA1001700.012.746315120
AGATCGG1113000.011.633425120
TTAGATC107100.010.832116120
CACACGT47100.010.616805120
ACACGTC56550.010.37537120
TAGATCG256400.010.295783114-115
CTAGATC60100.010.067243110-111
CACGTCT51200.010.027076120
ACGTCTG60100.09.98439120
GAGATCG427800.09.628773118-119
AAGATCG382800.09.562114120
Produced by FastQC (version 0.11.5)