FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12567717
Sequences flagged as poor quality0
Sequence length87-126
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT440950.09.210333120
AGATCTC435550.09.035285120
GAGTGTA644350.08.611838120
ATCTCGG429550.08.2816515120
GTGTAGA595000.08.267475120
GGTGGTC295650.08.177173120
AGTGTAG598050.08.125003120
AGAGTGT701100.08.077333120
TCTCGGT380400.07.900839120
AAGAGTG767400.07.887609120
TGTAGAT591750.07.735035120
CTCGGTG388200.07.6957297120
GTAGATC617300.07.686988120
GATCTCG488700.07.586162120
TCGGTGG341600.07.5513844120
TAGGGAA1085000.07.2540426120
AAAAAAA354350.07.110381120
TGGTCGC254000.07.0617685120
CGTATCA158100.07.019656120
AAAGAGT846050.06.9771013120
Produced by FastQC (version 0.11.5)