FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14860004
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC158050.073.19931120
GAGCACA258950.045.93531120
AGAGCAC340550.035.7877120
AAGAGCA530500.022.904678118-119
GAAGAGC603000.020.906109120
GGAAGAG764250.016.442905120
TCGGAAG879150.014.195591118-119
CGGAAGA908100.013.926086118-119
ATCGGAA1046900.012.4544525120
GATCGGA1062350.011.825827118-119
AGATCGG1147500.011.472675120
TAGATCG271100.010.717742116-117
CTAGATC48400.09.890866112-113
CCCCCCC6152.9353265E-49.4413541
GGGGGTA18200.08.9329731
GAGATCG500500.08.834251120
TTAGATC74750.08.450137116-117
CCCCCCA15001.4479156E-98.1290051
TCTAGAT27550.07.964244120
CAGATCG452900.07.868912120
Produced by FastQC (version 0.11.5)