FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11269308
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC153200.071.934120
GAGCACA246400.047.15942120
AGAGCAC313950.036.506134120
AAGAGCA462800.025.225325118-119
GAAGAGC526500.021.71357120
GGAAGAG621350.018.77113120
CGGAAGA713650.016.333235120
TCGGAAG730200.015.9729395118-119
CACGTCT48400.015.079924120
ATCGGAA865200.013.572893116-117
GATCGGA887200.013.309633116-117
CACACGT44600.012.962187120
AGATCGG996150.012.005946120
TAGATCG200300.011.328875110-111
ACACGTC53900.011.12788118-119
CTAGATC48500.010.579246110-111
ACGTCTG60650.010.24686120
TTAGATC70050.09.594291110-111
GAGATCG436150.09.47727118-119
AAGATCG311850.08.712951120
Produced by FastQC (version 0.11.5)