FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13446638
Sequences flagged as poor quality0
Sequence length66-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG13600.015.421619120
AGTCTAG20000.010.486701120
CTAGGGA10200.08.728874108-109
TGGTCGC269400.08.223147120
GTGGTCG294300.08.150978120
GGTCGCC269650.07.9599442118-119
TCTAGGG12500.07.952331116-117
TCTCGGT413700.07.905584120
CGTATCA194050.07.869755120
CTAGGTG14900.07.7952867114-115
CGGTGGT367900.07.767399120
GTCGCCG297900.07.6344523120
GCCGTAT236550.07.619538120
GGTGGTC325300.07.615995120
GATCTCG490500.07.50959120
AGATCTC498250.07.4585543120
TAGATCT512100.07.320827120
TCGGTGG375050.07.26981120
CGAGTAG23800.07.254415114-115
GTCTAGG13300.07.080865114-115
Produced by FastQC (version 0.11.5)