FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13446638
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC123800.058.834106120
GAGCACA192550.039.486774120
AGAGCAC248550.031.692013120
AAGAGCA402650.020.27558120
GAAGAGC422050.018.963802118-119
GGAAGAG532500.015.45384120
CGGAAGA601100.014.1241045120
TCGGAAG602250.014.086307120
GATCGGA719100.012.441177120
ATCGGAA720800.012.104252120
AGATCGG796200.011.144274118-119
TAGATCG200250.011.1365385120
TTAGATC89800.08.484904118-119
CTAGATC54500.08.375258120
CACGTCT44250.08.241612118-119
AAGATCG305300.08.06309116-117
GAGATCG315200.07.716865116-117
ACACGTC44950.07.5434546120
CACACGT36450.07.5039215116-117
CAGATCG299150.07.2803807120
Produced by FastQC (version 0.11.5)