FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14763419
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC167950.069.624176120
GAGCACA256600.047.19798120
AGAGCAC343550.034.594837118-119
AAGAGCA519800.023.493723120
GAAGAGC594150.020.57647120
GGAAGAG728800.016.750574118-119
TCGGAAG858850.014.211214120
CGGAAGA866150.014.082703118-119
ATCGGAA1016350.012.240917120
GATCGGA1030800.012.081153118-119
ACACGTC67150.011.234723120
AGATCGG1125150.011.206907120
TAGATCG285950.011.16551120
CACACGT61500.010.623974120
CACGTCT61150.010.464477120
CTAGATC69950.09.629476120
GAGATCG436500.09.05003112-113
TTAGATC111850.08.852617120
AAGATCG386800.08.474944116-117
CAGATCG382350.08.174237120
Produced by FastQC (version 0.11.5)