FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16739282
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC164550.072.29354120
GAGCACA265800.045.972065120
AGAGCAC354700.034.896564120
AAGAGCA553700.022.938929120
GAAGAGC640600.020.435175120
GGAAGAG793650.016.419529120
TCGGAAG944150.014.606294120
CGGAAGA950450.014.155249120
GATCGGA1101800.012.675539118-119
ATCGGAA1114050.012.657245120
AGATCGG1200950.011.329602118-119
TAGATCG319900.010.9577875120
CTAGATC70150.010.63392120
TTAGATC119550.08.803636118-119
GAGATCG490050.08.725301116-117
AAGATCG419100.08.383931116-117
CCTAGAT25550.08.2680235120
GCTAGAT26750.07.897121120
TCTAGAT33900.07.688619118-119
CAGATCG415400.07.5690637116-117
Produced by FastQC (version 0.11.5)