FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13269466
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC155450.059.82466120
GAGCACA232150.042.66263120
AGAGCAC308150.031.887537120
AAGAGCA479400.021.879473120
GAAGAGC526100.019.887901120
GGAAGAG638250.017.034777120
CGGAAGA751700.014.213776118-119
TCGGAAG756800.014.100139120
ATCGGAA906300.012.426762120
GATCGGA907550.012.223463120
AGATCGG1003900.011.341617120
TAGATCG257750.010.186243120
ACACGTC50500.09.394265120
CTAGATC60750.09.33527118-119
TTAGATC103800.08.866487112-113
CACACGT42500.08.768877118-119
GAGATCG374050.08.611757118-119
CACGTCT47650.08.31952120
AAGATCG386300.08.271557118-119
CAGATCG341200.07.7882504118-119
Produced by FastQC (version 0.11.5)