FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11552435
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC105200.053.691963120
GAGCACA148500.038.380157120
AGAGCAC194600.030.92791120
AAGAGCA314900.019.051348118-119
GAAGAGC336800.017.679909118-119
GGAAGAG426100.014.589123120
TCGGAAG471850.013.336892120
CGGAAGA496700.012.515451120
GATCGGA539650.011.530892118-119
ATCGGAA558250.011.295323118-119
AGATCGG590800.010.543157116-117
TAGATCG158500.09.986289120
CTAGATC36600.09.939752120
TTAGATC72050.08.326751120
AAGATCG260700.07.6380773118-119
GAGATCG247250.07.524256116-117
CCTAGAT16050.06.9587865118-119
CAGATCG208250.06.2827315110-111
CACGTCT34803.6379788E-126.0522437120
CGCTAGA7200.00204939025.761601114-115
Produced by FastQC (version 0.11.5)