FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12119772
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC89700.051.669464120
GAGCACA130800.036.94774120
AGAGCAC173050.030.43004120
GAAGAGC319650.017.345142120
AAGAGCA321050.016.922573120
GGAAGAG441750.014.24587120
TCGGAAG464250.014.168567120
CGGAAGA505900.012.659608120
AGATCGG611600.011.766757120
TAGATCG152900.011.614993120
GATCGGA589050.011.607927120
ATCGGAA596250.011.156414120
CTAGATC40950.010.27542120
TTAGATC77600.08.213346120
AAGATCG308500.08.013186118-119
GAGATCG239400.07.612106118-119
CCTAGAT20250.07.028253120
CAGATCG228200.06.385853118-119
ACACGTC29903.092282E-116.2086134120
TCTAGAT30250.05.932296108-109
Produced by FastQC (version 0.11.5)