FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13825637
Sequences flagged as poor quality0
Sequence length50-126
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC187650.0141.76201120
GAGCACA313200.082.61001120
AGAGCAC415100.063.072945118-119
AAGAGCA633650.039.72225118-119
GAAGAGC693000.037.616047116-117
GGAAGAG833650.030.076645116-117
CACGTCT51050.029.419964120
CGGAAGA966900.026.97405114-115
TCGGAAG989500.025.151785114-115
ACACGTC56800.023.236652120
ATCGGAA1156250.022.871605112-113
CACACGT49450.022.525133118-119
GATCGGA1184850.021.35537112-113
AGATCGG1279650.020.790396110-111
GCACACG66550.020.516209120
ACGTCTG65300.018.469563120
TAGATCG313050.017.592411110-111
CGTCTGA77000.017.5841120
GAGATCG529350.017.060678110-111
CTAGATC46650.016.08135108-109
Produced by FastQC (version 0.11.5)