FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15260326
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC158900.075.18706120
GAGCACA251750.046.531506120
AGAGCAC342550.034.91731120
AAGAGCA534400.023.061378120
GAAGAGC591500.020.643183118-119
GGAAGAG720100.016.899612118-119
CGGAAGA846100.014.544883116-117
TCGGAAG841800.014.444513116-117
ATCGGAA999300.012.376783114-115
GATCGGA991600.012.21771114-115
AGATCGG1080050.011.391555120
CTAGATC68100.011.198696110-111
TAGATCG264850.010.74171116-117
TTAGATC102100.08.775771110-111
ACACGTC48550.08.748286120
GAGATCG400450.08.444803118-119
CAGATCG452300.08.187608114-115
AAGATCG353150.08.055744118-119
CACACGT38950.07.725325118-119
GCTAGAT28500.07.198825114-115
Produced by FastQC (version 0.11.5)