FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15366294
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC152350.062.832542120
GAGCACA227050.042.075367120
AGAGCAC314150.033.013916120
AAGAGCA505500.021.472717120
GAAGAGC537200.019.719965118-119
GGAAGAG696250.015.90446120
TCGGAAG777550.014.606031120
CGGAAGA810700.014.175648120
GATCGGA954950.012.965253120
ATCGGAA942700.012.511895120
AGATCGG1036200.011.793209120
TAGATCG207150.010.915169120
CTAGATC54350.010.548695120
AAGATCG525550.09.235864118-119
GAGATCG355050.09.126131120
TTAGATC93900.08.472462118-119
CAGATCG345050.07.859742118-119
CCTAGAT21250.06.9726167112-113
CAAGATC312900.06.804081116-117
ACACGTC45150.06.7057652120
Produced by FastQC (version 0.11.5)