FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12798990
Sequences flagged as poor quality0
Sequence length53-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA1247550.014.643819120
GTATCAT599000.010.336604120
CGTATCA612450.010.246749120
GCGTCGG80850.09.943741120
TCATTAA464250.09.485695120
ATCATTA523600.09.304251120
GCCGTAT745000.09.204813120
CATTAAA453250.09.133481120
CCGTATC695000.09.042619118-119
GGTCGCC970850.08.985397120
CGCCGTA798750.08.915894120
TATCATT619900.08.797661120
GTCGCCG932000.08.529527120
TCGCCGT880550.08.4215120
TGGTCGC1025200.08.421262120
GTGGTCG1105650.08.23719120
GGTGGTC1204950.08.036362120
ATTAAAA535050.07.288598120
TCGGTGG1369450.07.2725534120
CTCGGTG1453600.07.2725377120
Produced by FastQC (version 0.11.5)