FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20866574
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC152100.053.025185120
GAGCACA226450.037.921345120
AGAGCAC288900.028.874823118-119
AAGAGCA500300.017.767857120
GAAGAGC519750.017.478174120
GGAAGAG642550.015.126725120
TCGGAAG717600.013.518401120
CGGAAGA759100.012.547298118-119
GATCGGA852500.011.903189120
ATCGGAA866600.011.477216120
AGATCGG949500.010.647417120
TAGATCG239250.010.070945120
CTAGATC57900.09.289898118-119
GAGATCG437250.07.380927120
TTAGATC106550.07.1442275120
CACACGT48200.07.048068120
AAGATCG354900.06.824614118-119
CAGATCG429150.06.640679120
ATAGATC154850.06.0533867120
TGAGATC183050.05.9800234120
Produced by FastQC (version 0.11.5)