FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11936437
Sequences flagged as poor quality0
Sequence length52-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA815100.013.1025095120
GCGTCGG105100.09.134034120
TCATTAA266000.08.887097120
TGGTCGC560750.08.527749120
CATTAAA265550.08.269517120
GGTCGCC550050.08.268228120
GTATCAT350850.08.242727120
CGCCGTA476200.07.950333120
CGTATCA356650.07.931939118-119
TATCATT357900.07.895953120
TCGGTGG757800.07.7354455120
GCCGTAT452300.07.7071695120
GTGGTCG611950.07.6523867118-119
ATCATTA299850.07.55358118-119
GGTGGTC655100.07.4644084120
CGGTGGT724600.07.2121572120
TCTCGGT828050.07.2096114120
TAGATCT1014650.07.178733120
GATCTCG946150.07.165786120
CCGTATC443250.07.1335754120