FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11936437
Sequences flagged as poor quality0
Sequence length50-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC156350.078.033165120
GAGCACA250800.049.72415120
AGAGCAC329550.037.529545118-119
AAGAGCA484500.025.468327118-119
GAAGAGC582150.021.99635120
GGAAGAG708600.017.800304118-119
CGGAAGA821900.015.631899120
TCGGAAG827100.015.499216120
ATCGGAA959000.013.582594120
GATCGGA996150.013.176029120
TAGATCG248900.012.07596118-119
AGATCGG1103550.011.903568118-119
CCTAGAT20050.011.708804120
CTAGATC54650.011.260193118-119
TTAGATC74450.010.320091116-117
CACGTCT55000.010.088925120
GAGATCG442700.09.689932120
CAGATCG417050.09.603582120
CACACGT53300.09.409831118-119
ACGTCTG65700.09.312066120
Produced by FastQC (version 0.11.5)