FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11508583
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC111900.062.049408120
GAGCACA173450.043.0533120
AGAGCAC236000.032.780174120
AAGAGCA367050.020.989882118-119
GAAGAGC426700.018.9692120
GGAAGAG510000.015.589198118-119
TCGGAAG590000.014.14153120
CGGAAGA606100.013.765885120
GATCGGA694100.013.43913120
ATCGGAA685950.012.582855120
AGATCGG778350.011.91874120
TAGATCG196650.011.249424118-119
CTAGATC48300.010.39132118-119
TTAGATC80850.09.529173118-119
AAGATCG323050.09.123876118-119
CCTAGAT16600.08.858449120
TCTAGAT27200.08.697025120
GAGATCG282150.08.690274118-119
CAGATCG253950.08.48437120
GCTAGAT21000.07.915748120
Produced by FastQC (version 0.11.5)